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Are you using wrong b12 ? . Know the difference ( this post is educational purpose only ) . Not all B12 forms are the same. Methylcobalamin and Cyanocobalamin both work, but
(attached, full text here: ) Windgassen, S
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Immunoblotting The dishes were washed 2× with ice-cold PBS, lysed in 1× Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%–12% Bis-Tris gels, after which the proteins were overnight transferred at 4°C to polyvinylidene fluoride membranes
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