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If you ever see visible particles, cloudiness, or gel-like material in your reconstituted peptide, aggregation has likely occurred

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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 μM 8-oxo-dGTP, 2 μM dPTP, 20 PCR cycles Library 2: 4 μM 8-oxo-dGTP, 1 μM dPTP, 20 PCR cycles Library 3: 8 μM 8-oxo-dGTP, 0.5 μM dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 × 10 7 for each library